rox reference dye (New England Biolabs)
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New England Biolabs
rox reference dye
Rox Reference Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rox+reference+dye/Luna+Universal+Probe+qPCR+Master+Mix/pm41373499-275-17-20
Average 99 stars, based on 742 article reviews
Rox Reference Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rox+reference+dye/Luna+Universal+Probe+qPCR+Master+Mix/pm41373499-275-17-20
Average 99 stars, based on 742 article reviews
rox reference dye - by Bioz Stars,
2026-09
99/100 stars
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CRISPR:Article Title: Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics Article Snippet: .. CRISPR-drCas12f1/Cas13a-based dual-gene detection This method is adapted from the “Trans-cleavage reporter assay” section, with modi cations to the reaction setup as follows: Cas13a protein (100 nM), Cas12f1/K173A protein (100 nM), Cas13a crRNA (100 nM), Cas12f1 sgRNA (100 nM), FAM/BHQ1-labelled RNA reporter (500 nM), VIC/BHQ1-labelled ssDNA reporter (500 nM), buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg/ml BSA), RNase inhibitor (4 U), Reporter Assay:Article Title: Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics Article Snippet: .. CRISPR-drCas12f1/Cas13a-based dual-gene detection This method is adapted from the “Trans-cleavage reporter assay” section, with modi cations to the reaction setup as follows: Cas13a protein (100 nM), Cas12f1/K173A protein (100 nM), Cas13a crRNA (100 nM), Cas12f1 sgRNA (100 nM), FAM/BHQ1-labelled RNA reporter (500 nM), VIC/BHQ1-labelled ssDNA reporter (500 nM), buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg/ml BSA), RNase inhibitor (4 U), Article Title: Harnessing noncanonical trans -cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics Article Snippet: .. This method is adapted from the “ Trans -cleavage reporter assay” section, with modifications to the reaction setup as follows: Cas13a protein (100 nM), Cas12f1/K173A protein (100 nM), Cas13a crRNA (100 nM), Cas12f1 sgRNA (100 nM), FAM/BHQ1-labelled RNA reporter (500 nM), VIC/BHQ1-labelled ssDNA reporter (500 nM), buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg/mL BSA), RNase inhibitor (4 U), Real-time Polymerase Chain Reaction:Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells Article Snippet: .. Triplicate reactions were used for each sample using the Luna Universal Probe qPCR 2× Master Mix with Article Title: A recombinant norrin-derived growth factor retains the complete multifunctionality of human norrin Article Snippet: .. Triplicate reactions were run for each sample using the Luna Universal Probe qPCR 2x Master Mix with Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells. Article Snippet: .. Triplicate reactions were used for each sample using the Luna Universal Probe qPCR 2× Master Mix with Amplification:Article Title: Harnessing noncanonical trans -cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics Article Snippet: .. This method is adapted from the “ Trans -cleavage reporter assay” section, with modifications to the reaction setup as follows: Cas13a protein (100 nM), Cas12f1/K173A protein (100 nM), Cas13a crRNA (100 nM), Cas12f1 sgRNA (100 nM), FAM/BHQ1-labelled RNA reporter (500 nM), VIC/BHQ1-labelled ssDNA reporter (500 nM), buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg/mL BSA), RNase inhibitor (4 U), |